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For samples that are thermolabile such as peptides, proteins and other samples of biological interest, soft ionization techniques are to be considered. For samples that are not themolabile and relatively volatile, ionization such as Electron Impact and/or Chemical Ionization can be effectively used. One of the main factor in choosing which ionization technique to be used is thermolability. Depending on the samples chemical and physical properties, different ionization techniques can be used. The instrument used could be considered as a sophisticated balance which determines with high precision the masses of individual atoms and molecules. Mass spectrometry consists basically of weighing ions in the gas phase. Qualitative and quantitative analysis of components in a mixture. Mass spectrometry is an analytical technique that involves the study in the gas phase of ionized molecules with the aim of one or more of the following: Separation Methods for Coupling with Mass Spec.Atmospheric Pressure Chemical Ionization.This tutorial discusses basic aspects of mass spectrometry that will be helpful to you in deciding the proper techniques and measurements for your research samples.